A Leader in the Development of Reagents for Wnt Research

R&D Systems Recombinant Mouse Wnt-3a has Higher Activity than Mouse Wnt-3a From Other Companies. A. The MC3T3-E1 mouse preosteoblast cell line was treated with increasing concentrations of R&D Systems Recombinant Mouse Wnt-3a (Catalog # 1324-WN) or with mouse Wnt-3a from two other companies. Bioactivity of the recombinant proteins was assessed by measuring alkaline phosphatase production. B. R&D Systems Recombinant Mouse Wnt-3a and recombinant mouse Wnt-3a from two other companies were tested for their ability to stimulate activation of the TOPflash beta-Catenin/TCF reporter in the HEK293T human kidney cell line. In both assays, the R&D Systems protein shows greater than a four-fold increase in activity compared to the other commercially available proteins.

R&D Systems Recombinant Mouse Wnt-3a is Purer than Mouse Wnt-3a From Other Companies. R&D Systems Recombinant Mouse Wnt-3a (Catalog # 1324-WN; lane 1, 1 μg/lane) and recombinant mouse Wnt-3a from two other companies  (lanes 2 & 3, 1 μg/lane) were loaded on a 12% SDS-PAGE gel under reducing conditions and visualized by silver staining.

R&D Systems Offers the First Commercially Available Recombinant Mouse sFRP-5. Recombinant Mouse sFRP-5 (Catalog # 7195-SF) was tested for inhibition of the activation of beta-Catenin in a TOPflash Luciferase Assay. In the presence of 50 ng/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN), increasing concentrations of sFRP-5 inhibited activation of the TOPflash beta-Catenin/TCF reporter in the HEK293T human kidney cell line.

R&D Systems Offers the First Commercially Available Recombinant Human Wnt-10b. Recombinant Human Wnt-10b (Catalog # 7196-WN) was tested for activation of cell proliferation. The IEC-18 rat small intestinal epithelial cell line was grown in the presence of increasing concentrations of Wnt-10b. Cell proliferation was assessed in a fluorometric assay using the redox sensitive dye Resazurin (Catalog # AR002).

A Literature Survey Reveals Wnt Researchers Choose R&D Systems Wnt Proteins More Than Any Other Wnt Protein Manufacturer. Research articles published from 2011 to present that cite the use of recombinant Wnt proteins were analyzed to determine the primary sources of the reagents. Of the 110 research articles that were identified, 88.2% utilized Wnt proteins manufactured by R&D Systems. Four other companies accounted for the additional 11.8% of articles identified in the survey.

Neutralization of R-Spondin 1-induced beta-Catenin Activation in a TOPflash Luciferase Assay. In the presence of 5 ng/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN), Recombinant Human R-Spondin 1 (Catalog # 4645-RS) stimulates activation of the TOPflash beta-Catenin/TCF reporter in the HEK293T human kidney cell line (blue line). The effect induced by 100 ng/mL Recombinant Human R-Spondin 1 was neutralized in a dose-dependent manner using a Sheep Anti-Human R-Spondin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4645; orange line).

Detection of Frizzled-1 in Mouse Intestine. Frizzled-1 was detected in a cryostat tissue section of embryonic mouse intestine (E13) using a Goat Anti-Mouse Frizzled-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1120). The tissue was stained using the Anti-Goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).

Detection of Intracellular beta-Catenin using the DuoSet^® IC ELISA Development System. The levels of beta-Catenin in lysates prepared from the MCF-7 human breast adenocarcinoma, HeLa human cervical epithelial carcinoma, A431 human epithelial carcinoma, SK-MEL-28 human malignant melanoma, and DLD-2C2 human colorectal adrenocarcinoma cell lines were assessed using the Human Total beta-Catenin DuoSet IC ELISA (Catalog # DYC1329; bar graph). The results of the DuoSet IC ELISA are consistent with the relative levels of beta-Catenin detected by Western blot analysis (inset).